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Title In vitro regeneration and micro-propagation of enset from Southwestern Ethiopia
Full text http://dx.doi.org/10.1023/A:1026701419739
Date 2001
Author(s) Negash, A.; Puite, K.; Schaart, J.; Visser, B.; Krens, F.
Abstract Three clones of enset (Ensete ventricosum Welw. Cheesman) from southwestern Ethiopia (Keffa-Shaka zone) were investigated for their potential for micropropagation and regeneration in tissue culture. Corm and leaf tissue of greenhouse-grown plants as well as in vitro germinated zygotic embryos were used as starting material for micro-propagation and regeneration studies. Embryos were excised from disinfected seeds and cultured in vitro. Multiple shoots from both corm- and embryo-explants were obtained using regeneration medium supplemented with 10 M or 20 M BAP. Rooting of shoots was achieved using medium with 5 M IBA, 1 M BAP and 1 g l-1 activated charcoal. Plantlets obtained by this process were transferred to soil under greenhouse conditions. Optimal conditions, which were determined for clonal propagation of three different genotypes of enset, allowing both in vitro micropropagation and regeneration, are described. This protocol makes for conservation of enset clones, rapid propagation of selected disease-free germplasm and more efficient breeding procedures.
Subject(s) Biodiversiteit en Veredeling
Relation http://library.wur.nl/WebQuery/wurpubs/314384
Type of publication Article/Letter to editor
Format unknown/unknown
Source Plant Cell, Tissue and Organ Culture 62: 153 - 158
Rights Wageningen UR
Repository Wageningen - University of Wageningen
Added to C-A: 2008-12-22;01:54:21